Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm.
Identifieur interne : 000293 ( Main/Exploration ); précédent : 000292; suivant : 000294Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm.
Auteurs : Anna Shaliutina [République tchèque] ; Martin Hulak ; Ievgenia Gazo ; Pavla Linhartova ; Otomar LinhartSource :
- Animal reproduction science [ 1873-2232 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Comet Assay (veterinary), DNA (chemistry), DNA (metabolism), DNA Damage, Endangered Species, Fishes (genetics), Fishes (physiology), Male, Oxidative Stress (genetics), Oxidative Stress (physiology), Protein Carbonylation, Semen Preservation (methods), Semen Preservation (veterinary), Sperm Motility (physiology), Spermatozoa (physiology), Thiobarbituric Acid Reactive Substances (metabolism).
- MESH :
- chemical , chemistry : DNA.
- chemical , metabolism : DNA, Thiobarbituric Acid Reactive Substances.
- genetics : Fishes, Oxidative Stress.
- methods : Semen Preservation.
- physiology : Fishes, Oxidative Stress, Sperm Motility, Spermatozoa.
- veterinary : Comet Assay, Semen Preservation.
- Animals, DNA Damage, Endangered Species, Male, Protein Carbonylation.
Abstract
The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s(-1) in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TBARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species.
DOI: 10.1016/j.anireprosci.2013.03.006
PubMed: 23602487
Affiliations:
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Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Hulak, Martin" sort="Hulak, Martin" uniqKey="Hulak M" first="Martin" last="Hulak">Martin Hulak</name></author><author><name sortKey="Gazo, Ievgenia" sort="Gazo, Ievgenia" uniqKey="Gazo I" first="Ievgenia" last="Gazo">Ievgenia Gazo</name></author><author><name sortKey="Linhartova, Pavla" sort="Linhartova, Pavla" uniqKey="Linhartova P" first="Pavla" last="Linhartova">Pavla Linhartova</name></author><author><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23602487</idno><idno type="pmid">23602487</idno><idno type="doi">10.1016/j.anireprosci.2013.03.006</idno><idno type="wicri:Area/PubMed/Corpus">000254</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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last="Shaliutina">Anna Shaliutina</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Vodnany, Czech Republic. a_shalutyna@mail.ru</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Hulak, Martin" sort="Hulak, Martin" uniqKey="Hulak M" first="Martin" last="Hulak">Martin Hulak</name></author><author><name sortKey="Gazo, Ievgenia" sort="Gazo, Ievgenia" uniqKey="Gazo I" first="Ievgenia" last="Gazo">Ievgenia 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scheme="MESH" xml:lang="en"><term>Animals</term><term>DNA Damage</term><term>Endangered Species</term><term>Male</term><term>Protein Carbonylation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s(-1) in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TBARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Gazo, Ievgenia" sort="Gazo, Ievgenia" uniqKey="Gazo I" first="Ievgenia" last="Gazo">Ievgenia Gazo</name><name sortKey="Hulak, Martin" sort="Hulak, Martin" uniqKey="Hulak M" first="Martin" last="Hulak">Martin Hulak</name><name sortKey="Linhart, Otomar" sort="Linhart, Otomar" uniqKey="Linhart O" first="Otomar" last="Linhart">Otomar Linhart</name><name sortKey="Linhartova, Pavla" sort="Linhartova, Pavla" uniqKey="Linhartova P" first="Pavla" last="Linhartova">Pavla Linhartova</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Shaliutina, Anna" sort="Shaliutina, Anna" uniqKey="Shaliutina A" first="Anna" last="Shaliutina">Anna Shaliutina</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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